L-Serine quantification by ELISA?
L-Serine quantification by ELISA?
Now possible!
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L-Serine
(L-Ser) is a non-essential amino acid
which becomes conditionally essential - in certain circumstances -
particularly when it cannot be synthesized in a sufficient amount to
meet the cellular demands. L-Ser may be derived from different sources
including dietary intake, the glycolytic intermediate
3-phosphoglycerate, glycine as well as protein and phospholipid
degradation. It’s worth noting that enzymes involved in L-Ser
biosynthesis are upregulated in highly proliferating cells, including cancer cells, to limit
L-Ser over-consumption. Besides its essential role in cell
proliferation, L-Ser is important for brain development and functions
which are especially sustained by i) altered L-Ser levels in psychiatric disorders
and ii) occurrence of severe neurological
abnormalities in patients with
defects in L-Ser biosynthesis.
Given its
roles in such critical functions, L-Ser quantification in biological
samples is critical. We thus developed and validated a novel Serine ELISA kit (#IS-I-1200)
which allows for L-Ser quantitation in biological samples including
plasma, sera as well as cell culture supernatants. The assay requires a
low sample volume of 10μL and is characterized by a 2.4µM sensitivity.
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Sensitive
& easy-to-implement
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Typical standard
curve obtained with the Ser ELISA kit (#IS-I-1200) where the optical
density is inversely correlated with L-Ser concentration within a range
of 3.8 - 625μM.
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Cross-validated
with LC/MS
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L-Ser was quantified
in human plasma samples from 40 subjects using IS-I-1200 ELISA
kit and by LC/MS. Correlation between both methods gave a
R-squared value above 0.95, thereby confirming the accuracy of the
immunoassay.
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Special release offer
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Valid until October 15, 2021
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