2-5151-000
Strep-Tactin®XT 4Flow® high capacity Spin Column Kit
Please be informed that we have replaced
the Strep-Tactin®XT Spin Column Kit (2-4151-000) by our new improved
kit with higher protein binding capacity, Strep-Tactin®XT 4Flow® high
capacity Spin Column Kit.
Strep-Tactin®XT 4Flow® high capacity Spin
Column Kit is perfectly suitable for rapid purification of Strep-tag®II and Twin-Strep-tag® fusion
proteins from multiple small samples in parallel. The kit contains ready-to-use
wash buffer as well as elution buffer, Strep-Tactin®XT 4Flow® high capacity
resin (50% suspension) and empty spin columns. The spin columns are not prefilled with
resin to allow a customer-specific application and experimental design. If the
spin columns are filled with, e.g., 100 µl Strep-Tactin®XT 4Flow® high
capacity resin (50 µl column bed volume) up to 600 µg protein can be purified.
The frit (mesh size 10 µm) in the lower part of the empty spin columns ensure
that the resin remain in the spin column while sample or buffer flow through
rapidly during the centrifugation process. The spin columns are compatible with
conventional 1.5 ml or 2.0 ml reaction tubes. The maximum filling volume is 700
µl.
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