CABRTM Bacterial Probes
Since
the microbial specificity of periodontal diseases is well
established, having a valid microbial diagnostic test is essential
for a correct and a coherent treatment planning. DNA probe will be
used to identify and quantify the oral pathogens, most commonly associated with
periodontitis. Oligodeoxynucleotide probes have been developed for
identification of the periodontal bacteria Actinobacillus actinomycetemcomitans,
Bacteroides gingivalis,
B. intermedius types I and II, B. forsythus, Eikenella corrodens,
Fusobacterium nucleatum, Haemophilus aphrophilus,
Streptococcus intermedius, and Wolinella recta.
FISH Probes were
designed by sequencing the 16S rRNA for each bacterium, identifying
hypervariable regions, and chemically synthesizing
species-specific probes. These probes were specific when tested
against a panel of nucleic acids from closely related bacteria.
Our
specialists from Creative Bioarray have
developed a more rapid identification assay using species- or
genus-specific probes. Creative Bioarray is
one of the well-recognized experts who is professional in FISH technologies for
a broad range of project objective. With years of experience, our scientists
can offer high-quality FISH analysis of microorganisms to meet your demands.
Fig
1. Methanolobus spp. (orange) and other members of Archaea(green) in CIB-1
Fig
2. FISH detection of Wolbachia (Yellow) on FFPE sections of testes of insects;
CRC specimen triply stained with DAPI, EUSO and EUB338. Cell nuclei (in
blue), bacterial conserved regions (in green) and F. nucleatum specific regions (in red)
Fig
3. 2 kinds of bacterial FISH probes to detect
all bacteria and Porphyromonas gingivalis
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