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A validated L-Phenylalanine ELISA kit
now available for testing!
L-Phenylalanine (Phe) is an essential amino acid that cannot be synthetized by humans or other vertebrates and must be supplied from an exogenous diet. Phe is transformed to L-Tyrosine (Tyr) through the Phenylalanine hydroxylase (PAH) enzyme and thus serves as a precursor of key catecholamines including Dopamine, Noradrenaline and Adrenaline. Mutations in the gene encoding for PAH have been linked to Phenyloketonuria (PKU), an aminoacidopathies - associated with fair skin, eczema, seizures, tremors, and hyperactivity - characterized by an increased blood/brain level of Phe and its derivatives, and a concomitant decrease in Tyr. Besides its known role in PKU, Phe has been recently linked to severity of COVID-19 where higher level of Phe has been detected for patients with moderate/severe disease in comparison to patients with asymptomatomatic/midly SARS-COV-2 infection (Atila et al, Amino Acids, 2021) thus shedding light on role of Phe on the control of immune response.
In order to provide accurate and easy-to-implement tool to better understand Phe functions, we developed and validated a novel L-Phenylalanine ELISA kit (#IS-I-1700) which allows for Phe quantitation in biological samples including plasma and sera. The assay requires a sample volume as low as 20μL and is characterized by a 1.7µM sensitivity.
Sensitive & easy-to-implement
Typical standard curve obtained with the Phe ELISA kit (#IS-I-1700) where the optical density is inversely correlated with Phe concentration within a range of 3.4 - 390μM.
Cross-validated with LC/MS
Phe was quantified in human plasma samples from 40 subjects using IS-I-1700 ELISA kit and by LC/MS. Correlation between both methods gave a R-squared value above 0.99, thereby confirming the accuracy of the immunoassay.
Special release offer
Valid until November 30, 2021
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