mCherry is a monomeric cherry
fluorescent protein related to DsRed, a red fluorescent protein
from Discosoma disc corals. The mCherry protein is widely used in
biological research, including as a tracer in the imaging of mCherry-expressing live cells,
validating the expression of a transgene, and studying the behaviors of
specific cell types in vivo.
More recently, the advent of antibodies to mCherry has further advanced its
research use. Antibodies to visualize mCherry
protein expression have been particularly useful in neuroscience research,
especially in localizing the customized mCherry expressing neuronal cell lines,
virally infected natural cells, or in highlighting cell-specific mCherry transgene expression. (PMID:
28228637, PMC5768486, PMC9547883)
three types of antibodies from Chicken, Rabbit, and Mouse, specifically
designed to recognize mCherry
blots (WB) and in sections through immunohistochemistry (IHC).
monoclonal antibody (#M-1653-100) Chicken
#M-1653-100: IF analysis of HEK293 cells
transfected with mCherry protein construct, red, and stained mouse anti-mCherry antibody, M-1653-100, green. Transfected cells that overlap mCherry protein and anti-mCherry antibodies appear golden in color. Untransfected cells appear only to show their
DAPI-stained blue nuclei.
IF: The confocal microscopical images show the HEK293 cells transfected
with mCherry (red) and stained with C-1655-100 (green). The transfected mCherry (red) with chicken anti-mCherry
antibody (green) together appear golden. Untransfected
cells appear blue from a nuclei-only stain. The chicken anti-mCherry antibody provides a strong and
WB: It shows HEK293 mCherry expressing cell lysates using
chicken pAb to mCherry,
C-1655-100, in green, and rabbit pAb to GAPDH, R-1701-100, in red: Lanes:  MW Markers,  untransfected HEK293 control cells,  HEK293
cells expressing two tdTomato protein domains,  HEK293
cells expressing mCherry-HA protein, and  HEK293
expressing GFP protein. C-1655-100 mCherry
antibody recognizes tdTomato and mCherry proteins revealing major bands
at about 60kDa and 30kDa but does not recognize GFP. The red band at 37kDa
corresponds to GAPDH protein, used as a loading control.
analysis of HEK293 cells transfected with mCherry
protein construct, red,
and stained with rabbit pAb to
mCherry, R-1654-100, green.
Nuclear DNA staining is blue.
protein glow golden in
this analysis, as expected. The R-1654-100
antibody shows only golden-colored transfected cells expressing mCherry
protein and untransfected
cells, which reveal only their nuclei
(주)엘바이오시스템은 biosensis의 한국공식대리점입니다.
제품의 주문 및 관련정보가 필요하시면 전화, 이메일로 문의해 주세요.