Description
Neuropeptide
Y (NPY) is a member of a regulatory peptide family and has a marked sequence
homology with pancreatic polypeptide (PP) and peptide YY (PYY), which are other
members of the family. NPY is widely expressed in the nervous system and
has been shown to be differentially expressed in inhibitory interneurons in the
hippocampus in degenerative disease, as a powerful vasoconstrictor in the
periphery, and increased expression of NPY in the hypothalamus correlates with
food intake.
In
the rat central nervous system, immunohistochemistry has found NPY-like cell
bodies in the cortex, caudate-putamen, hypothalamus (arcuate nucleus),
hippocampus, anterior westolfactory
bulb, nucleus accumbens,
amygdaloid complex and periaqueductal grey. NPY-like fibers and terminals are
detected in high numbers in the bed nucleus of the stria terminalis, the peri-
and paraventricular regions of the hypothalamus and thalamus and in discrete
hypothalamic nuclei, particularly the suprachiasmatic nucleus.
Westerns
are not recommended for the Neuropeptide Y antibody since the protein is too
small to detect in gel.
Photo
Description: IHC image of neurons staining for
neuropeptide Y in the rat cortex (above) and striatum (below). The tissue was
fixed with 4% formaldehyde in phosphate buffer, before being removed and
prepared for vibratome sectioning. Floating sections were incubated at RT in
10% goat serum in PBS, before standard IHC procedure. Primary antibody was
incubated at 1:5000 for 48 hours, goat anti-rabbit secondary was subsequently
added for 1 hour after washing with PBS. Light microscopy staining was achieved
with standard biotin-streptavidin/HRP procedure and DAB chromogen.
The second image above entitled “C.” is NPY interneurons (RED) in mouse
striatum, GREEN staining is GFP expressed by medium spiny neurons provided Alipi
Naydenov, Neurobiology and Behavior,
Stella Lab University of Washington (see review below).
Host: Rabbit
Quantity / Volume: 100 µL
State: Lyophilized Whole Serum
Reacts With: Alpaca (Llama), Bird, Bubalus
Bubalis (Water Buffalo), Bullfrog, Cat, Ewe, Ferret, Fish, Frog, Frog (Xenopus
Laevis), Ground Squirrel, Guinea Pig, Hamster, Human, Monkey, Mouse,
Ovine (Sheep), Pig, Psetta Maxima (Flat Fish), Rat, Sheep, Snake, Tadpole,
Toad, Turtle, Zebrafish
Availability: In Stock
Preabsorption Control: Available
at Sigma Aldrich, Catalog # N3266
Alternate Names: Pro-neuropeptide Y;
Neuropeptide tyrosine; prepro-neuropeptide
Y; PYY4, anti-NPY
Gene Symbol: NPY
