Strep-Tactin® Superflow® high capacity resin
50%
suspension to purify Strep-tag®II
or Twin-Strep-tag® fusion proteins
2-1208-002 / 2-1208-010 / 2-1208-025 / 2-1208-500
Description
Strep-Tactin® Superflow® high capacity is a resin for
purification of strep-tagged proteins <90 kDa
via affinity
chromatography.
The product is optimized for column-based use and suitable for HPLC/FPLC. It
consists out of highly cross-linked agarose beads that are coupled to Strep-Tactin®, which is a streptavidin variant. Strep-Tactin® binds to proteins that were fused
to a Strep-tag®II or a Twin-Strep-tag®, enabling a straightforward purification
of tagged proteins from mixed samples, reaching a purity of >95%. Due to the
targeted elution, tedious titration steps are not necessary. In addition,
regeneration allows for multiple
use of the resin.
In comparison to Strep-Tactin® Superflow, the high
capacity version has a higher density of
Strep-Tactin®, making it suitable for highly expressed
proteins. This resin is also an alternative for proteins that are difficult to
elute from our Strep-Tactin®XT 4Flow® resins.
Please
note that biotinylated proteins also bind to Strep-Tactin® Superflow® high capacity. If biotinylated
proteins are expected, use BioLock. Furthermore, this resin is a 50%
suspension, so for packing e.g. a 1 ml gravity column, 2 ml of resin is
required.
For
further information about our different resins including recommendations, check
our resin
specifications.
Specifications
|
Bead
Size:
|
60
- 160 µm spherical
|
|
Biotin
Binding Capacity:
|
>
900 nmol/ml resin
|
|
Dynamic
Protein Binding Capacity:
|
7
mg/ml resin
|
|
Exclusion
Limit:
|
6 x
10^6
|
|
Form:
|
50%
suspension
|
|
Possible
Application:
|
Protein
purification of Strep-tag®II
and Twin-Strep-tag® fusion proteins
|
|
Specificity:
|
Twin-Strep-tag®; Strep-tag®II; cross reactivity: biotin
|
|
Support:
|
6%
agarose, crosslinked
|
Shipping
information
|
Storage:
|
2-8
°C
|
|
Stability:
|
6
months after shipping
|
|
Shipping:
|
Room
temperature
|
