We
are excited to introduce you to AnkyronsTM a breakthrough in target binding technology that overcomes many of the
current constraints of research antibodies. Unlike traditional antibodies, AnkyronsTM are based on an ankyrin-repeat
scaffold. These small (~15 kDa), single binding domain proteins
revolutionize the research landscape and depart from the animal-immune response
paradigm of antibodies. But what sets AnkyronsTM apart?
AnkyronsTM are selected in
vitro by
ribosome display from ProImmune's synthetic TeralibraryTM of over 1 trillion (1012) Ankyron
clones for medium to high affinity to their target. The exceptionally large
number of clones in our TeralibraryTM makes selecting Ankyrons significantly more efficient
than selecting antibody fragments from comparable antibody screening libraries in
vitro.
As synthetic reagents, Ankyrons are defined, recombinant,
monoclonal sequences by default.
Finding
murine-specific antibodies can be a challenge. We have already generated Ankyrons against a wide variety of murine
proteins tested in immunofluorescence microscopy. Below is Ankyron staining with a mouse p53
specific Ankyron. p53 plays a crucial role in
preserving genomic integrity by preventing genome mutation and is a key player
in cellular responses to stress, controlling several genes involved in cell
cycle arrest, DNA repair, apoptosis, and cellular metabolism.
Figure:
Mouse
macrophage J774A.1 cells were permeabilised with 0.5% Triton X-100, fixed in
formaldehyde (4%), then stained using 1.4µg V5-tagged Ankyron and anti-V5 antibody conjugated
with APC (red) in Permeabilisation Staining Buffer (RPMI 1640 + 10
% human serum + 0.5 % saponin), counterstaining nuclei with DAPI (blue) and
F-actin with Phalloidin (green). Image captured by epifluorescence microscopy.
Inset: negative control of anti-V5 APC antibody alone and F-actin staining.
Watch a 5 minute podcast presented by
our scientists to learn more about the AnkyronsTM
Ankyros™ Features:
·Single, highly-stable, small 15kD
binding domain
·Always recombinant, sequence
defined by default
·More than 1700 catalog target
specificities; multiple clones per target typically; rapidly expanding range
·No more primaries: get any Ankyron with any of our tags or
conjugates affordably to order
·No Fc- / Isotype background
·Selected entirely in
vitro
from our TeralibraryTM for higher affinity
·Unconstrained by immunological
tolerance
·Highly thermostable
·Custom recombinant monoclonal
generation for any budget*
·Wide range of formatting options
for multi-specifics and multivalent molecules
·More cost-effective than research
antibody technology in every respect
·Generated without animal
immunization
·Distinguish closely-related
proteins with Ankyron guided selection
·For research applications, including: immunofluorescence, flow
cytometry, ELISA, immunohistochemistry, immunoprecipitation, cell culture and
more
Guided selection
What's
more, using a guided selection strategy in our discovery process, we can easily
screen Ankyron binders for selective reactivity
with one type of target over
closely related versions of the same target.
Prominent examples include peptide-specific Ankyrons
to peptide-MHC molecules and idiotype specific Ankyrons
to
antibodies, such as drug antibodies.
Figure: Mouse macrophage J774A.1 cells
were stained using 1.4 µg V5-tagged Ankyron and anti-V5 antibody conjugated
with APC (red) in Staining Buffer (RPMI 1640 + 10 % human serum),
counterstaining nuclei with DAPI (blue). Image captured by epifluorescence
microscopy. Inset: negative control of anti-V5 APC antibody alone.
Our rapidly growing catalog currently contains over 7,500 clones for
more than 1,500 target proteins and further to these catalog Ankyrons we have already generated, we
can do custom Ankyron generation.
View Our Ankyron Catalog
Comparing AnkyronsTM to antibodies side by side
