CytoSoft^® - New T-25 and T-75 Flasks

Substrates of Varying Rigidity

CytoSoft^® T-25 and T-75 flasks provide an increased surface area for passaging cells on an in vivo-like substrate. 

Learn more about the T-25 or T-75 flasks.

The CytoSoft^® portfolio includes a broad range of formats designed for different phases of research, including:

6-well plates - Initial discovery

24 and 96-well plates - High resolution imaging / publication quality data

T-25 and T-75 flasks - Cell passaging / expansion

"Knowing your cells grow best on an 8 kPa surface, for example, means nothing if researchers continue to passage cells on plastic dishes. This is why we created the CytoSoft^® T-Flasks." 

David Bagley, President, Advanced BioMatrix

The rigidity of the substrate to which cells adhere can have a profound effect on cell morphology, migration, differentiation and gene expression.

CytoSoft^® products provide a novel substrate for culturing cells on various rigidities, covering a broad physiological range. 

The unique use of biocompatible silicone substrates (vs. polyacrylamide) provides many benefits, including:

1. Longer shelf-life

2. Stiffness doesn't significantly change over time

3. Accurate stiffness measurements for each lot

4. Ships immediately (not made-to-order)

CytoSoft^® plates can also be used to show how fibroblasts are able to discriminate between the underlying stiffness.

This is manifested in both adhesion size and stress fibers, as seen below. It appears that the cells on the 8 kPa stiffness have reduced intracellular tension and increased adhesion. 

Re-create in vivo. Click Here to choose a substrate stiffness.