Strep-Tactin®XT 4Flow® high capacity resin

50% suspension to purify Strep-tag®II or Twin-Strep-tag® fusion proteins

  

Content

    

Strep-Tactin®XT 4Flow® high capacity is perfectly suited for purifying Strep-tag®II or Twin-Strep-tag® fusion proteins. This resin offers excellent pressure stability, pH compatibility, and target purity (> 95%). Additionally, this resin is compatible with a broad range of reagents.
Its high binding affinity makes it ideal for low abundant, high-expressing and challenging proteins. A low concentration of agarose also facilitates the purification of large proteins (>90 kDa).  When working with, for example, multimeric proteins, choosing a resin with the Strep-Tactin® ligand is recommended.
Strep-Tactin®XT 4Flow® high capacity resin has a higher density of immobilized Strep-Tactin®XT than the classic Strep-Tactin®XT 4Flow®and can thus can bind more protein.
As the resin is optimized for column affinity chromatography, rather than batch purification, the suspension can be used to pack gravity flow columns.

Specifications

Data sheet Strep-Tactin®XT 4Flow® high capacity resin

Protocol protein purification with Strep-Tactin®XT gravity flow columns

Protocol packing of a gravity flow column

Protocol expression of recombinant proteins in E. coli

Compatible reagents

Compatible reagents for Strep-Tactin®XT

Video

Packing of a Gravity Flow Column for Protein Purification

Color based functionality check - Strep-Tactin® and Strep-Tactin®XT columns compared

If you want more information, check out our resin specification overview.

 

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