Fluorescent
dyes - Activated
esters
AF 430 NHS ester
NHS esters AF 430 Protein labeling
AF 430 is a hydrophilic dye. It is used in flow cytometry and other applications.
The NHS ester functional group allows to conjugate the fluorophore efficiently with primary and secondary amine groups in both aqueous buffers and anhydrous conditions.
Absorption and emission spectra
of AF 430
General properties
|
Appearance:
|
yellow
powder
|
|
Molecular weight:
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701.75
|
|
CAS number:
|
467233-94-9
|
|
Molecular formula:
|
C32H42N3F3O9S
|
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Solubility:
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good
in water, DMF, DMSO
|
|
Quality control:
|
NMR 1H, HPLC-MS (95%)
|
|
Storage conditions:
|
Storage:
12 months after receival at -20°C in the dark. Transportation: at room
temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
|
|
MSDS:
|
Download
|
|
|
Product
specifications
|
Spectral properties
|
Excitation/absorption maximum, nm:
|
430
|
|
ε, L⋅mol−1⋅cm−1:
|
15955
|
|
Emission maximum, nm:
|
542
|
|
Fluorescence quantum yield:
|
0.23
|
|
CF260:
|
0,59
|
|
CF280:
|
0,24
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