Strep-Tactin®XT 4Flow® high capacity
Description
Strep-Tactin®XT 4Flow® high capacity is a 4% agarose coupled with the streptavidin variant
Strep-Tactin®XT and applicable for purification of Strep-tag®II or Twin-Strep-tag® fusion proteins. Due to the broad pH compatibility,
excellent pressure stability, outstanding target purity (> 95%), and highest
binding affinity, the resin is recommend for all protein classes, especially
for low abundant or challenging proteins. The low concentrated agarose enables
the simple attachment of larger proteins to the stationary phase, making it
particularly suitable for purification of high-molecular-weight targets. In
addition, Strep-Tactin®XT 4Flow® high capacity allows the application of denaturing
agents such as urea. In comparison to Strep-Tactin®XT 4Flow®, Strep-Tactin®XT 4Flow® high capacity has a higher density of immobilized
Strep-Tactin®XT, which leads to a 2-fold higher yield of purified protein.
The suspension can be used for packing of gravity flow
columns since the resin is optimized for column affinity
chromatography, rather than for batch purification. To pack a column with 1
ml resin bed volume, 2 ml 50% suspension are necessary.
Specifications
Bead Size: 50 - 150 µm spherical
Dynamic Protein Binding Capacity: 14 mg/ml resin
Form: 50% suspension
Possible Application: Protein purification of Strep-tag®II
and Twin-Strep-tag® fusion proteins
Specificity: Twin-Strep-Tag® and
Strep-tag®II
Support: 4% agarose, crosslinked
Shipping
information
Storage: 2-8 °C
Stability: 6 months after shipping
Shipping: Room temperature
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