XT is a streptavidin variant, which binds to the Strep-tag®II and Twin-Strep-tag®
with high affinity and specificity. It is available as conjugate with fluorescent dyes (DY-488, DY-549, DY-649) for fast and directed detection of N-terminally, C-terminally as well as internally tagged Strep-tag®
II or Twin-Strep-tag®
fusion proteins in applications
like ELISA, FACS, immunohistochemistry, and immunocytochemistry.
The absorption/emission maxima of the fluorescent dyes are:
• DY-488: 493/518 nm
• DY-549: 550-560/575 nm
• DY-649: 655/676 nm
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