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Flow cytometry Cell proliferation Microscopy Cell membrane
The ready-to-use kit contains all
necessary reagents to label cell membranes with PKH26 dye for evaluating
cell migration or proliferation.
The kit contains PKH26
cyanine dye with lipophilic groups, which allow rapid and non-covalent
incorporation into cell membranes of almost any cells without influence
on cell receptors or transmembrane proteins. The cells preserve
their biological properties and ability to proliferate, which makes PKH26
an excellent fluorophore for studying plant and animal cells in vivo and in vitro.
It can also be used for studying membrane vesicles.
PKH26 is a red
fluorescent dye with an emission maximum at 567 nm. It can
be used with other dyes in multicolor assays. PKH26 is rather
stable, and PKH26-labeled cells can be studied by microscopy or flow
cytometry within 100 days after staining.
The number of measurements
using the kit can vary depending on the cell count in the sample:
Examples of staining
Absorption and emission spectra