Assessing the polyamine pathway
are molecules derived from ornithine (Orn)
(i.e., putrescine, spermine, spermidine) or arginine (Arg) (i.e., agmatine).
They are present in mammalian cells at millimolar concentrations (1).
Key regulatory steps of their metabolism are represented by ornithine
decarboxylase (ODC), acetyl-CoA:spermidine/spermine
and membrane transport systems. While ODC is responsible for ornithine
degradation and spermidine production, cSAT
and nSAT transform spermidine to N1- and
N8-acetylspermidine (N1- and N8-AcSpdn), respectively. These molecules are
involved in many critical processes such as cell proliferation, nucleic acid
synthesis, and cytoprotection
from oxidative stress (2).
Several studies have evidenced that polyamine metabolism is frequently
increased in cancer (3), which
can fuel tumor progression (4) and
favor immune escape (5).
Elevated levels of polyamines in sera of cancer patients have been demonstrated
to correlate with poor prognosis (6).
new ELISA kits
the importance of this metabolic pathway in cancer progression (4) and
resistance to treatment (7),
investigating it requires robust tools. To this aim, we developed and validated
the first ELISA kits to quantify ornithine (#IS-l-1000R),
and N8-acetylspermidine (#IS-I-2500R) in
samples of different natures (plasma, cell culture supernatants) and species
Our catalog of small molecule
antibodies & ELISA kits
Our catalog only features
validated small molecule antibodies & ELISA kits. Selected for their
specificity and affinity by ELISA, our antibodies enable rapid & robust
detection and/or quantification of small molecules in various types of biological
materials, including cells and lysates, tissues and homogenates, culture
supernatants, plasma and serum samples.