Agarose Particles for Purification
of
Proteins and Antibodies
What
are Agarose Particles?
Agarose
particles are microspheres serving as a porous gel to filter or separate a
mixture of molecules according to their individual sizes. They are easy to be
activated, so that they can be prepared to bind biomolecules in a reversible or
irreversible manner. They can be used in batch or column purification of
proteins and antibodies, which are extremely suitable for biopharmaceutical
applications. The large internal porosity enables them to purify larger
biomolecules like monoclonal antibodies. Their particle distribution mainly
ranges from 20 to 300 μm.
Small particle size is propitious for polishing steps with extremely high
resolution, while the larger particle size is adapted for capture steps.
Besides, the volumes required also have an impact on the choice of particle
sizes. Large volumes usually need particles with larger particle size. There
are different percentages of agarose particles such as 2%, 4% and 6%, which
allows better separation of molecules based on their size. Particles with
higher agarose percentage are suitable to separate small globular proteins due
to their smaller pore size. In the same way, particles with lower percentage of
agarose are better to separate larger globular proteins. What's more,
pre-activated agarose particles can be used to bind biomolecules. The particles
coupled with proteins and antibodies on the surface can be used as affinity
chromatography resins, ion exchange chromatography resins, size-exclusion
chromatography resins, and hydrophobic interaction chromatography resins. The
enzyme-immobilized agarose particles can be used for antibody fragmentation.
CD
Bioparticles provides an extremely wide range of agarose particles mainly for
the isolation and purification of proteins and antibodies. These products are
classified into Basic
Agarose Particles, Functional
Agarose Particles and Conjugated
Agarose Particles for further search.
Scanning
electron micrographs of agarose particles. Adapted from Nweke, Mauryn
C, 2017.
Advantages
of Agarose Particles
•Particle sizes ranging from 25 to 500 μm.
•Various modifications for diverse
applications.
•With different capabilities and
suitable for different flow rates to match application requirements.
•Shortened purification cycles and
improved production efficiency.
•Agarose percentage: 2%, 4%, 6%,
7.5%.
Applications
of Agarose Particles
The
agarose particles from CD Bioparticles can be used in the isolation and
purification of proteins and antibodies. Examples of common applications are
below.
•Isolation and purification of
proteins with affinity tags.
•Isolation and purification of
antibodies.
•Preparation of antibody fragments.
•Conjugation of proteins, antibodies
and peptides.
•Used in pull-down assays, chromatin
immunoprecipitation assays and immunoprecipitation assays.
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