Strep-Tactin® HRP
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Description
Strep-Tactin® Sepharose® 50
% suspension can be used for the purification of Strep-tag®II or Twin-Strep-tag® fusion
proteins. The resin is particularly suited for the purification of proteins
with multiple tags, such as multimers, that cannot be eluted from Strep-Tactin®XT
resins because of the increased affinity. It can be used for the purification
of large proteins, due to the low percentage of agarose. Strep-Tactin® irreversibly
binds biotin, so if free biotin is expected use BioLock.
The suspension format allows the packing of gravity flow columns with custom
resin volumes. For a 1 ml resin bed volume, use 2 ml 50 % suspension.Strep-Tactin® Sepharose® is
not compatible with HPLC/FPLC due to a low pressure
stability.For
information on our other resins, please check the resin
specifications.
Specifications
|
Bead
Size:
|
45
- 165 µm
|
|
Biotin
Binding Capacity:
|
>
300 nmol/ml resin
|
|
Exclusion
Limit:
|
3 x
10^7
|
|
Form:
|
50%
suspension
|
|
Possible
Application:
|
Protein
purification of Strep-tag®II
and Twin-Strep-tag® fusion proteins
|
|
Specificity:
|
Twin-Strep-tag®; Strep-tag®II; cross reactivity: biotin
|
|
Strep-tag®II Binding Capacity:
|
3
mg/ml
|
Shipping information
|
Storage:
|
2-8
°C
|
|
Stability:
|
6
months after shipping
|
|
Shipping:
|
Room
temperature
|
