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[iba LifeSciences] Strep-Tactin® Sepharose® resin

관리자 2024.03.25 11:05 조회 165 추천 0
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Strep-Tactin® HRP





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Description


Strep-Tactin® Sepharose® 50 % suspension can be used for the purification of Strep-tag®II or Twin-Strep-tag® fusion proteins. The resin is particularly suited for the purification of proteins with multiple tags, such as multimers, that cannot be eluted from Strep-Tactin®XT resins because of the increased affinity. It can be used for the purification of large proteins, due to the low percentage of agarose. Strep-Tactin® irreversibly binds biotin, so if free biotin is expected use BioLock. The suspension format allows the packing of gravity flow columns with custom resin volumes. For a 1 ml resin bed volume, use 2 ml 50 % suspension.Strep-Tactin® Sepharose® is not compatible with HPLC/FPLC due to a low pressure stability.For information on our other resins, please check the resin specifications.


Specifications


Bead Size:

45 - 165 µm

Biotin Binding Capacity:

> 300 nmol/ml resin

Exclusion Limit:

3 x 10^7

Form:

50% suspension

Possible Application:

Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins

Specificity:

Twin-Strep-tag®; Strep-tag®II; cross reactivity: biotin

Strep-tag®II Binding Capacity:

3 mg/ml



Shipping information


Storage:

2-8 °C

Stability:

6 months after shipping

Shipping:

Room temperature






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