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[Intact Genomics] Your Next Level DNA Amplification : ig® Recombinase Polymerase Amplification (RPA) Kit

관리자 2024.01.16 11:45 조회 226 추천 0

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 ig® Recombinase Polymerase Amplification (RPA) Kit 





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IG® Recombinase Polymerase Amplification (RPA) Kit provides a mixture of the necessary enzymes to simplify and quicken the setup for your RPA reaction.


 



Description

The ig® RPA Kit from Intact Genomics gives the user a faster, more streamlined Recombinase Polymerase Amplification experience by providing the necessary reagents and enzymes to amplify user-provided DNA template. Isothermal RPA is an enhancement over PCR amplification because it does not require thermocycling and provides better sensitivity.  


Recombinase Polymerase Amplification (RPA) is a molecular biology technique that amplifies DNA at a constant temperature (37-42°C) using a recombinase (e.g. UvsX), primers, a single-stranded DNA binding protein (SSB), and a strand displacing DNA polymerase. T4 UvsX is used in combination with its accessory protein, UvsY. The recombinase interacts with the primers to form nucleoprotein filament. This complex can bind with homologous double-stranded DNA through a strand exchange. After the exchange, a single-stranded binding protein, T4 gp32, stabilizes the displaced strand. Finally, Bsu/Sau DNA polymerase extends the DNA from the primers and creates a new complete copy of the template. Amplification can continue as in Polymerase Chain Reaction (PCR).


Components and Storage

Enzyme Mixture
2x RPA Reaction Master Mix

280 mM Magnesium Acetate (MgOAc)

Storage Temperature

-20°C


Schematic of Recombinase Polymerase Amplification


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Related Products

1.T4 UvsX Recombinase (Cat.# 3562)
2.T4 gp32 Protein (Cat.# 3515)
3.T4 UvsY Protein (Cat.# 3572)
4.Bsu DNA Polymerase (Cat.# 3585)
5.Sau DNA Polymerase (Cat.# 3595)
6.Exonuclease III (Cat.# 3415)

7.Exonuclease IV (Nfo) (Cat.# 3425)

Technical Support

Intact Genomics is committed to supporting the worldwide scientific research community by supplying the highest quality reagents. Each new lot of our products is tested to ensure it meets the quality standards and specifications designated for the product.


Please follow the instructions carefully and contact us if additional assistance is needed. We appreciate your business and your feedback regarding the performance of our products in your applications.



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