SPARCL™[1] – A Simpler and Faster
ELISA Alternative
SPARCL™
(Spatial Proximity Analyte Reagent Capture Luminescence) assays use two
specific antibodies; one conjugated to HRP, the other to acridan, a
chemiluminescent substrate. When HRP and acridan conjugated antibodies bind to
their target biomarker they are brought into close proximity. Upon addition of hydrogen peroxide
(trigger solution), HRP catalyzes oxidation of proximal acridan molecules
causing a flash of chemiluminescence that is proportional to biomarker
concentration. Acridan molecules distant from HRP are not oxidized and therefore
produce no luminescence. This principle allows development of a range of rapid
and sensitive homogeneous immunoassays.
A typical SPARCL™ assay requires no wash
steps, uses a single incubation, and takes approximately 45 minutes start to
finish. This contrasts with ELISA’s that require 1-4 incubations, 1-3 wash
steps and take 2-4+ hours. SPARCL™ kits require a luminometer capable of
simultaneous injection and measurement. Click here to find out if your luminometer is
compatible. Click here for answers to SPARCL FAQs.
Currently available SPARCL™ kits are
listed below. Click on the catalog numbers for the kit instructions.
A list of publications that have used our
SPARCL™ assays can be found at the bottom of this page.
SPARCL™ Literature References
1.Baka
R, et al. Quantitative proteomics of cerebrospinal fluid using tandem mass tags
in dogs with recurrent epileptic sezures. J Proteomics 231:1039777
(2021) 4.Dalanezi FM, et al. Concentrations of acute phase
proteins in milk from cows with clinical mastitis caused by different
pathogens. Pathogens 9(9):706
(2020) 5.Boulay
I, et.al. SPARCL™: Use of a novel technology in validation of a Non-human
primate and rat cardiac troponin-I assay in serum. CiToxLAB,
2016
6.Boulay
I, et.al. SPARCL™: Use of a Novel Technology in Validation of a Non-Human
Primate C-Reactive Protein Assay in Serum. CiToxLAB.
2016
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